rabbit anti β 3 ar  (Alomone Labs)

Journal: Journal of Nanobiotechnology

Article Title: Aminolysis-mediated single-step surface functionalization of poly (butyl cyanoacrylate) microbubbles for ultrasound molecular imaging

doi: 10.1186/s12951-024-02806-9

Figure Lengend Snippet: In vitro binding of cRGD-MB to HUVEC under flow conditions. A Fluorescence images of non-stimulated (control) or TNF-α stimulated HUVEC. The nuclei, cell membrane and α v β 3 integrins were stained with DAPI (blue), wheat germ agglutinin-488 (WGA, green) and CD51/61 antibody (red), respectively. Scale bar = 50 μm. B Quantitative analysis indicating significantly higher area fraction of α v β 3 integrin in HUVEC stimulated with TNF-α. Data are represented as mean ± SD. C Schematic showing the in vitro setup of the flow chamber. Petri dishes with cultured HUVEC were connected to a flow chamber, in turn perfused with an MB solution (standard-MB, cRAD-MB, or cRGD-MB) at 0.25 mL/min. D Representative fluorescent images of the three different, rhodamine-labeled MB types binding to TNF-α stimulated HUVEC. The nuclei, cell membrane and MB were labeled with DAPI (blue), WGA (green) and rhodamine (red), respectively. Scale bar: 100 μm. E Number of bound MB per cell. cRGD-MB displayed significantly higher binding to TNF-α stimulated HUVEC compared to both controls. Data represent mean ± SD of three independent MB batches. Statistical comparisons were performed using unpaired t-tests in panel B, while for panel E comparisons were performed using one-way ANOVA. **p < 0.01 and ***p < 0.001

Article Snippet: The tumor sections were fixed with 80% methanol for 5 min at 4 °C followed by the addition of acetone at − 20 °C for 2 min. After fixation, sections were washed 3 times with PBS and incubated overnight with a rabbit anti-α v β 3 integrin antibody (1 μg/ μL; eBioscience, San Diego, California, USA) at a dilution of 1:100 at 4 °C.

Techniques: In Vitro, Binding Assay, Fluorescence, Control, Membrane, Staining, Cell Culture, Labeling

Journal: Journal of integrative neuroscience

Article Title: Evaluation of the Expansion and Neuronal Differentiation Potency of Cultured Olfactory Epithelium Progenitor Cells from a Rat Model of Depression.

doi: 10.31083/j.jin2302027

Figure Lengend Snippet: Fig. 4. OE progenitor cells derived from depressive-like rats showed reduced potential for neuronal differentiation. (A) Relative mRNA expression of β-3 tubulin. (B) Representative immunoblotting of β-3 tubulin. (C) Relative band intensity showing protein

Article Snippet: After blocking for one hour, the membrane was incubated separately with primary antibodies against β-3 tubulin (1:1000; #5568, Cell Signaling Technology, Danvers, MA, USA) or β-actin (1:1000; #4970, Cell Signaling Technology).

Techniques: Derivative Assay, Expressing, Western Blot